The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies

Fern is one of the groups primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, anti-hyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid abundantly present ferns. This study aims isolate major compounds found Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition lipoxygenase decrease production leukotriene induces bronchoconstriction asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from orientale. Further silico was performed explain binding mode between flavonoid pyranoside or galactoside aglycone 15-LOX cavity their amino acid residues interaction. binds with Ile663, Ile400, Leu408, Leu597, Ala404, Arg403 a inhibitor. Trifolin same acids isoquercetin addition His366, Gln596, Phe175. Both isoquercitrin competitive inhibitors against enzymes.